An enterokinase in the gut of pharate adult of Glossina morsitans morsitans Westwood (Diptera: Glossinidae).

نویسندگان

  • R M Vundla
  • D L Whitehead
چکیده

An enterokinase (Enteropeptidase, EC. 3.4.21.9) has been described in the pharate adult of Glossina mositans morsitans. The enzyme is present in pharate adults, 21 days after pupation. It activated commercial crystalline bovine trypsinogen to trypsin. It showed affinity for concanavalin A bound to sepharose and was reversibly sensitive to boiling at pH 6.0. The apparent molecular weight, as determined by gel permeation on sepharose 6B-CL, suggests self-aggregation or an association with a large molecule (M.Wt. approximately equal to 2.5 X 10(6)).

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The effects of a DNA virus infection on the reproductive potential of female tsetse flies, Glossina morsitans centralis and Glossina morsitans morsitans (Diptera: Glossinidae).

Reproductive anomalies associated with the tsetse DNA virus infection in the female tsetse hosts, Glossina morsitans centralis Machado and Glossina morsitans morsitans Westwood, inoculated with the virus during the 3rd instar larval stage were studied and the data compared to those obtained from the control females injected with sterile physiological saline. Virus infected flies had significant...

متن کامل

A comparison of three types of "M" traps for sampling tsetse fly (Diptera: Glossinidae) populations at south Luangwa game management area, Zambia.

A field trial was conducted between 27th July and 1st August, 1992 in Mfuwe, south Luangwa, Zambia to assess the effectiveness of locally developed "M" traps for suppressing and sampling the tsetse flies Glossina pallidipes Austen and Glossina morsitans morsitans Westwood. The tsetse catches in the "M" traps and the standard F3 traps were collected at 24 hour intervals. The highest catches of b...

متن کامل

Repellent Properties of δ-Octalactone Against the Tsetse Fly, Glossina morsitans Morsitans

Delta-octalactone, produced by several Bovidae, has been suggested as a potential repellant of tsetse fly attack. Racemic delta-octalactone was synthesized via an abbreviated route. The product was assayed against 3-day old starved teneral female tsetse flies, Glossina morsitans morsitans Wiedemann (Diptera: Glossinidae), in a choice wind tunnel and found to be a potent tsetse repellent at dose...

متن کامل

Digestive processes of haematophagous insects. XI. Partial purification and some properties of six proteolytic enzymes from the tsetse fly Glossina morsitans morsitans Westwood (Diptera: Glossinidae).

By 0-(diethylaminoethy1)cellulose (DEAE-cellulose) chromatography, affinity chromatography, and Sephadex gel filtration, six proteolytic enzymes active in alkaline medium have been found in the digestive portion of the midgut (tissue and lumen contents) of adult Glossina morsitans morsitans. By use of synthetic substrates the enzymes have been characterized as aminopeptidase (AP; EC. 3.4.11.1),...

متن کامل

The effect of starvation on the susceptibility of teneral and non-teneral tsetse flies to trypanosome infection.

Transmission of vector-borne diseases depends largely on the ability of the insect vector to become infected with the parasite. In tsetse flies, newly emerged or teneral flies are considered the most likely to develop a mature, infective trypanosome infection. This was confirmed during experimental infections where laboratory-reared Glossina morsitans morsitans Westwood (Diptera: Glossinidae) w...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Acta tropica

دوره 42 1  شماره 

صفحات  -

تاریخ انتشار 1985